Table I. Dissociation constants obtained from fluorescence polarization assays. The total binding equation was used to calculate dissociation constants with Graphpad Prism software. Kd values were tabulated from two independent experiments (performed in triplicate) and averaged. Error represents the S.D. of two independent experiments.
| Pdp3 | Peptide | Kd(μm) |
|---|---|---|
| WT | H3K36me3 | 69.5+/−3.7 |
| WT | H3K36me2 | 414+/−23 |
| WT | H3K36me1 | ≫1000 |
| WT | H3K36me0 | ≫1000 |
| WT | H3K4me3 | ≫1000 |
| WT | H3K79me3 | 434+/−49 |
| WT | H4K20me3 | ≫1000 |
| F18A | H3K36me3 | 667+/−68 |
| W21A | H3K36me3 | 703+/−73 |