Fig. 1.

Lewis blood groups detected in 10 human milk samples by glycan microarray analysis. The neutral HMG mixtures from 10 human milk samples were printed on NHS-activated slides at 200 μm as quantified by total fluorescence using LNnT-AEAB as a standard. Four defined AEAB-labeled glycans were printed at 100 μm to serve as controls: Le^a, Galβ1–3(Fucα1–4)GlcNAcβ1–3Galβ1–4Glc; H1, Fucα1–2Galβ1–3GlcNAcβ1–3Galβ1–4Glc; Le^b, Fucα1–2Galβ1–3(Fucα1–4)GlcNAcβ1–3Galβ1–4Glc; and LNT, Galβ1–3GlcNAcβ1–3Galβ1–4Glc. The array was assayed with anti-Le^a antibody (1 μg/ml), anti-H type 1 antibody (1:10 dilution), and anti-Le^b antibody (10 μg/ml).