Fig. 2.

Structures of glycans comprising the glycan targets selected to identify the milk glycan determinants bound by VP8*N155. The glycan targets were selected from a TGL of HMG based on microarray screening of the corresponding HMG-SGM with the recombinant VP8* domain of spike protein VP4 from human neonatal rotavirus strain N155(G10P[11]) as described in the companion report (Yu et al.²). The detailed structural determinations by MSⁿ are described in the supplemental materials. Linkages assigned by MSⁿ analyses are indicated in green and those assumed based on being from milk glycans are indicated in blue. Trivial names of previously described glycans are indicated based on recent reviews (7, 10, 24), and NPI indicates not previously identified as a free glycan in human milk.