Fig. 6.

Growth of bacteria lacking malate synthase (glyoxylate shunt) or glyoxylate carboligase (glycerate pathway) on different carbon substrates. A–C, bacterial strains were wild type (WT), malate synthase-deficient (ΔglcB), glyoxylate carboligase-deficient (Δgcl), or deficient in both enzymes (ΔglcB Δgcl). The double mutant strain was complemented with a single copy attB-integrating plasmid encoding glyoxylate carboligase (ΔglcB Δgcl attB::pgcl). Bacteria were grown on M9 minimal medium containing glucose (A) or acetate (B and C) as the sole carbon source. Growth was monitored by measuring the optical densities of the cultures at 600 nm (A_{600 nm}). Symbols and error bars represent mean values and ranges (n = 2 independent experiments).