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. 2014 Jul 20;14:195. doi: 10.1186/s12870-014-0195-5

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Copyright © 2014 Gubert et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Expression of theHAE::GUSabscission zone marker is displaced inas1medial sepals. Images of wild-type and mutant flowers (stage 17) histochemically stained for β-Glucuronidase (GUS) activity. Medial (A-D) and lateral (E-H) views are shown. A/E: In wild-type flowers, HAE regulatory regions direct expression of GUS in discrete domains of the remaining sepal (se), petal (pe) and stamen (st) abscission zone cells. B/F: In as1-20 flowers, GUS is expressed in an inverted V-shaped proximal region of the medial sepals (B), and in the remaining petal and lateral sepal stubs (B/F). C/D/G/H: Expression of GUS expands beyond the enlarged sepal abscission zones of as1-20 bp-1(C/G) and bp-1(D/H) flowers into the distal pedicels. Scale bars: 200 μm.