Table 4. Primers used for frequency examination and allele-specific PCR.
Sequencing primer | |||
---|---|---|---|
No. | name | Primer sequence | Length of products (bp) |
1 | F-3679 | GAT ACG AGC CAT TCT GTG T | 530 (containing mutation E1338D) |
R-4208 | TGC CAT TCT GTT GAC CTG | ||
2 | F-13512 | CCC ACG GAA GAG GAG AAA | 791 (containing mutation Q4594L and I4790M) |
R-14302 | TCC AGT TTA CGG GCT ATC T | ||
3 | F-14596 | ACA ACT CGT TCC TAT ACT CTC | 152 (containing mutation G4946E) |
R-14747 | TGT TTC CCG TTA TGC GTG AC | ||
Allele-specific primer | |||
4 | F-3979-S | ATG AAG GAG GCA GCT CAA | 116 (for E1338D genotyping) |
F-3979-R | ATG AAG GAG GCA GCG CAT | ||
R-4361 | TCC TCT TGA CCA TCA TCA TAG T | ||
5 | F-13620-S | GGA AGA TAA TGG ACA AGT TCA | 168 (for Q4594L genotyping) |
F-13620-R | GGA AGA TAA TGG ACA AGT TCT | ||
R-13787 | GCT TGC TTC TTA GCT TGT TC | ||
6 | F-14677-S | TGT TGG ACG TGG CTG TAG G | 71 (for G4946E genotyping) |
F-14677-R | TGT TGG ACG TGG CTG TAG A | ||
R-14747 | TGT TTC CCG TTA TGC GTG AC |
The differences of susceptible and resistant allele specific nucleotides were underlined, and the mismatched nucleotides were marked in bold.