Table 4. Primers used for frequency examination and allele-specific PCR.

Sequencing primer
No.	name	Primer sequence	Length of products (bp)
1	F-3679	GAT ACG AGC CAT TCT GTG T	530 (containing mutation E1338D)
	R-4208	TGC CAT TCT GTT GAC CTG
2	F-13512	CCC ACG GAA GAG GAG AAA	791 (containing mutation Q4594L and I4790M)
	R-14302	TCC AGT TTA CGG GCT ATC T
3	F-14596	ACA ACT CGT TCC TAT ACT CTC	152 (containing mutation G4946E)
	R-14747	TGT TTC CCG TTA TGC GTG AC
Allele-specific primer
4	F-3979-S	ATG AAG GAG GCA GCT CAA	116 (for E1338D genotyping)
	F-3979-R	ATG AAG GAG GCA GCG CAT
	R-4361	TCC TCT TGA CCA TCA TCA TAG T
5	F-13620-S	GGA AGA TAA TGG ACA AGT TCA	168 (for Q4594L genotyping)
	F-13620-R	GGA AGA TAA TGG ACA AGT TCT
	R-13787	GCT TGC TTC TTA GCT TGT TC
6	F-14677-S	TGT TGG ACG TGG CTG TAG G	71 (for G4946E genotyping)
	F-14677-R	TGT TGG ACG TGG CTG TAG A
	R-14747	TGT TTC CCG TTA TGC GTG AC

The differences of susceptible and resistant allele specific nucleotides were underlined, and the mismatched nucleotides were marked in bold.