Table 1.

Primer sequences

Gene	Sequence	Size (bp)
ALP	Forward (1397–1416): CCACGTCTTCACATTTGGTG	196
	Reverse (1573–1592): AGACTGCGCCTGGTAGTTGT
COL1A1	Forward (3982–4001): GAGAGCATGACCGATGGATT	178
	Reverse (4140–4159): CCTTCTTGAGGTTGCCAGTC
COL10A1	Forward (1670–1690): AATGCCTGTGTCTGCTTTTAC	130
	Reverse (1779–1799): ACAAGTAAAGATTCCAGTCCT
GAPDH	Forward (113–133): TGAAGGTCGGAGTCAACGGAT	181
	Reverse (273–293): TTCTCAGCCTTGACGGTGCCA
GLI1	Forward (676–695): AAGCGTGAGCCTGAATCTGT	189
	Reverse (845–864): CAGCATGTACTGGGCTTTGA
GLI2	Forward (199–218): CGACACCAGGAAGGAAGGTA	203
	Reverse (382–401): TGCACAGAACGGAGGTAGTG
GLI3	Forward (2285–2304): CTTTGCAAGCCAGGAGAAAC	163
	Reverse (2428–2447): TTGTTGGACTGTGTGCCATT
IHH	Forward (519–538): CGGCTTTGACTGGGTGTATT	219
	Reverse (718–737): AAAATGAGCACATCGCTGAA
OC	Forward (20–39): TGAGAGCCCTCACACTCCTC	151
	Reverse (170–151): CGCCTGGGTCTCTTCACTAC
OPN	Forward (759–778): TGAAACGAGTCAGCTGGATG	162
	Reverse (920–901): TGAAATTCATGGCTGTGGAA
PTC1	Forward (1460–1479): TCAGCAATGTCACAGCCTTC	248
	Reverse (1688–1707): GTCGTGTGTGTCGGTGTAGG