Figure 2.

The Foxd1+ Cortical Stroma Is a Progenitor Population for Stromal Tissues in the Kidney

Confocal immunofluorescence images of Foxd1^GC/+; R26R^lacZ/+ kidneys at 15.5 dpc with anti-β-gal staining (green).

(A–D) Nephrogenic zone.

(A) FOXD1 (red).

(B) SIX2 (red). Black arrows indicate β-gal+ SIX2+ cap mesenchyme cells.

(C) KRT (red).

(D) LIV2 (red). White and black arrows indicate inner LIV2+ β-gal+ cells in the renal capsule and outer LIV2+ β-gal− cells in the connective tissue, respectively.

(E and F) Renal cortex with glomeruli.

(E) PDGFRB (red) and WT1 (blue). Yellow and white arrows indicate PDGFRB+ β-gal+ pericytes and mesangial cells, respectively.

(F) FLK1 (red) and WT1 (blue). Yellow and white arrowheads indicate FLK1+ β-gal− vascular cells in the renal cortex outside of the glomerulus and within the glomerular tuft, respectively. Black arrowheads in (E) and (F) indicate WT1+ β-gal+ podocytes.

(G and H) Renal medulla.

(G) CDKN1C (P57KIP2, red) and KRT (white).

(H) F4/80 (red).

(I) Ureter with SMA (red).

Scale bars, 50 μm.