Figure 4.

Six2+ Cap Mesenchymal Cells and Foxd1+ Cortical Stromal Cells Differentiate in a Coordinated Manner

Kidneys at 19.5 dpc from Six2^GCE/+; Foxd1^GCE/+; R26R^lacZ/+ mice with oil-only injection at 14.5 dpc (A, E, I, M, Q, and U), Six2^GCE/+; R26R^lacZ/+ with 6 mg tamoxifen at 14.5 dpc (B, F, J, N, R, and V), Foxd1^GCE/+; R26R^lacZ/+ with 6 mg tamoxifen at 14.5 dpc (C, G, K, O, S, and W), and Six2^GCE/+; Foxd1^GCE/+; R26R^lacZ/+ with 6 mg tamoxifen at 14.5 dpc (D, H, L, P, T, and X).

(A–D) β-gal-stained (blue) whole-mount kidney.

(E–P) β-gal-stained (blue) sections counterstained with eosin (pink).

(E–H) Nephrogenic zone and renal cortex. Black arrows and white arrows indicate capillary loop-stage and maturing-stage glomeruli, respectively.

(I–L) Capillary loop-stage glomerulus.

(M–P) Maturing-stage glomerulus.

(Q–X) Confocal immunofluorescence images with anti-β-gal (red), a podocyte marker anti-NPHS1 (nephrin, green), and a mesangium marker anti-PDGFRB (white).

(Q–T) Capillary loop-stage glomerulus. Yellow arrowheads and yellow arrows in (R)–(T) indicate the β-gal+ NPHS1+ podocytes and β-gal+ PDGFRB+ mesangial cells, respectively.

(U–X) Maturing-stage glomerulus.

a, adrenal gland; ci, renal cortical interstitium; k, kidney; ms, mesangium; nt, nephron tubule; pe, parietal epithelium; pr, pericyte; ve, visceral epithelium (podocyte). Scale bars, 500 μm (A–D), 100 μm (E–H), and 25 μm (I–X).