Figure 6.
Foxd1+ Cortical Stromal Cells Can Be Multipotent Stromal Progenitors
(A and B) Models for developmental potential of Foxd1+ cells.
(A) A Foxd1+ cortical stromal cell retains multipotency to differentiate into different stromal tissues.
(B) Developmental potential of a Foxd1+ cell is restricted to limited stromal cell types within the Foxd1+ cortical stroma.
(C–G′) β-gal-stained (blue) kidneys from Foxd1GCE/+; R26RlacZ/+ embryos at 19.5 dpc after injection of oil only (C and E), and 0.1 mg tamoxifen (D, F, G, and G′) at 12.5 dpc.
(C and D) Whole-mount view. The inset in (D) shows a higher magnification of a clone in the dashed region. The arrow in (D) indicates a β-gal+ clone.
(E and F) Sections counterstained with eosin (pink).
(G and G′) Adjacent sections 96 μm apart showing the same clone. (G) is a higher magnification of the dashed region in (F). White arrows in (G) indicate multiple β-gal+ cells in the cortical stroma.
a, adrenal gland; ci, renal cortical interstitium; cm, cap mesenchyme; cs, cortical stroma; k, kidney; mi, renal medullary interstitium; ms, mesangium; np, nephron precursor; nt, nephron tubule; ut, ureteric tip. Scale bars, 500 μm (C and D), 200 μm (E and F), and 50 μm in (G and G′).