Figure 4.

Functionality of iSCs in Coculture with Neuronal Cells

(A) Culture of NSC-derived neurons alone, on cell tracker-labeled fibroblasts and iSCs, respectively. Scale bars, 100 μm.

(B) NSC-neurons cultured with iSCs form a more dense and branched network analyzed by MAP2-stained area, neurite number, and total neurite length (n = 3). ^∗p < 0.05.

(C) Coculture of iSCs with primary rat DRG neurons. Some iSCs form single myelinated fragments (arrowhead) detected by colocalization of MBP staining (yellow) and neurofilament (NF) staining (magenta). Scale bars, 20 μm.

See also Figure S4.