Figure 3.

Treatment with Transformed hBMSCs Does Not Protect against Experimental Colitis In Vivo

Colitis was induced by intracolonic administration of TNBS (3 mg/mouse dissolved in 50% ethanol). Mice (n = 10 per group) were treated i.p. with the indicated hBMSCs (10⁶), 8 hr after TNBS injection. Control mice received 50% ethanol.

(A and B) Clinical evolution was monitored by body weight changes and survival (A) as well as measuring the colitis score (B).

(C) Colon length and weight and macroscopic colonic damage score were evaluated at day 4.

(D) Histopathology was determined 4 days after cell infusion (four mice per group). No peritoneal tumors derived from transformed hBMSCs were observed. The scale bar represents 200 μm. ^∗p value < 0.001 versus TNBS colitic mice (two-way ANOVA); ^#p value < 0.001 versus BMSC-0H-treated mice (paired Student’s t test).