Figure 1.

Derivation of HD-NPCs from iPSCs and In Vitro Neural Differentiation of HD-NPCs

(A) Schematic diagram depicting NPC and neuron derivation from rhesus macaque iPSCs.

(B) Bright-field image of HD-14 NPCs at progressing stages of differentiation. Arrows indicate neural rosettes (first row). Immunocytochemistry of HD-14 NPCs stained for Nestin, PAX6, MSI1, and SOX2 (red; second row). HD-14 in vitro differentiated neurons stained for β-III tubulin, MAP2, GABA, and TH (red; third row). Neural receptor subunits are expressed in HD neural cells; NR2A (red), GluR1 (red), and mGlur5 (red) colabeled with β-III tubulin (green) (fourth row). Nuclear staining is shown using Hoechst (blue).

(C) FACS analysis of HD-14 NPCs using antibodies for NPC markers. FACS data for all cell lines are listed in Table S2.

(D) Quantification of neurons derived from in vitro differentiated NPCs. Error bars indicate the SEM. Data represent three biological replicates.

See also Figures S1 and S2 and Table S2.