Figure 2.

Characterization of Neuron Differentiation in Grafted NPCs

(A and B) Twelve and a half weeks posttransplantation, WT and HD cell grafts were positive for DCX (red).

(C and D) Fifteen weeks posttransplantation, WT and HD cell grafts were positive for GABA (red).

(E and F) Fifteen weeks posttransplantation, WT and HD cell grafts were positive for DARPP32 (red).

(G and H) Sixteen weeks posttransplantation, WT cell grafts were negative for mEM48; HD cell grafts show colocalization of GFP (green) and mEM48-positive nuclear inclusions (red).

(I and J) Twelve and a half weeks posttransplantation, WT cell grafts express neuronal marker NeuN (red). NeuN-positive HD cell grafts were observed after 16 weeks grafting (red).

(K and L) WT and HD cell grafts differentiate to glial cells indicated by positive GFAP staining (red).

Arrows indicate marker-positive transplanted neurons. Scale bars, 10 μm.