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. 2014 Jul 15;13:105. doi: 10.1186/1475-2840-13-105

Figure 6.

Figure 6

High-concentration glucose induces the transactivation of β-catenin-responsive genes. A, Glucose (10–30 mM, 24 h), PMA (50 nM, 8 h), and LiCl (10 mM, 8 h) induced an increase in TOPflash but not mutant FOPflash luciferase activity in human umbilical vein endothelial cells (HUVECs). Three independent experiments were performed. Luciferase activity was normalized to that of Renilla and is expressed as a percentage of the control. B, C, Western blot analysis showing that the treatment of HUVECs with glucose (24 h), LiCl (10 mM, 16 h) or PMA (16 h) increased protein expression of cyclin D1. D, Real-time PCR analysis showing that the treatment of HUVECs with glucose (10–30 mM, 24 h), LiCl (10 mM, 8 h), or PMA (50 nM, 8 h) increased mRNA expression of urokinase plasminogen activator, u-PA but not the endothelial cell marker CD31. Expression levels for the u-PA gene were normalized to those of the housekeeping gene β2-microglobulin. Data are expressed as the mean ± SD of 4 independent experiments. ***P < 0.001; **P < 0.01, and *P < 0.05 vs. control. Each experiment was independently performed 3 to 4 times.