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. 2014 Jul 21;11:130. doi: 10.1186/1743-422X-11-130

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Copyright © 2014 Shridhar et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Role of TAR in CAF suppression of HIV-1 LTR. Schematic of the TAR from HIV-1 LTR with the arrows indicating the sites at which it was cleaved, and the shadow, the intervening region that was deleted (Panel A). Reporter gene product (CAT) expression in cells pretreated with Hanks buffer (-CAF) or conditioned culture medium from a transformed CD8+ T cell line (+CAF) and transfected with the BstdelTAR construct, containing 3 SpI sites and TATA box (Panel B) Results are representative from at least 2 independent experiments conducted in triplicate. *, p < 0.05; ns, not significant.