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. 2014 Jul 21;11:130. doi: 10.1186/1743-422X-11-130

Figure 4.

Figure 4

Role of Sp1 sites in CAF suppression of transcription of HIV-1 LTR. Suppression of reporter gene product (luciferase) expression in cells pretreated with Hanks buffer (-CAF) or conditioned culture medium from a transformed CD8+ T cell line (+CAF) and transfected with a construct containing only the 3Sp1 sites and TATA box of HIV-1 LTR. (Panel A) Reporter gene product (CAT) expression in cells pretreated with Hanks buffer (-CAF) or conditioned culture medium from a transformed CD8+ T cell line (+CAF) and transfected with Sp1 deletion constructs (Panel B) or SpI mutated constructs (Panel C). The position of the specific Sp1 either deleted or mutated is given by the number on the x axis. The sequence in Panel C shows the bases (in bold) that were mutated to inactivate the SpI sites (underlined). CAF-primary refers to conditioned culture media from primary CD8+ T cells from an HIV infected individual Results are representative from 2 independent experiments conducted in triplicate. *, p < 0.05.