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. 2014 Jul 21;11:130. doi: 10.1186/1743-422X-11-130

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Copyright © 2014 Shridhar et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Transcription of TATA-TAR construct is suppressed by CAF. Reporter gene product (CAT) expression in cells pretreated with Hanks buffer (-CAF) or conditioned culture medium from a transformed CD8+ T cell line (+CAF) and transfected with a construct containing only the TATA box and TAR from HIV-1 LTR, in the presence or absence of the HSV-1 ICP0 protein. Transcription from the LTR was driven either by CMV-Tat (Panel A) or addition of PMA (Panel B) Results are representative of 2 independent experiments conducted in triplicate. *, p < 0.05.