Table 1.

SpI deletion construct oligos and primers

S No	Name of construct	DNA oligos used
1	Del 1, del 2	5’TCGGAGGACAGTACTCCGACCCGGTCGAAGGGAGGCGTGGCCTGAGCCCTCAGATCCTGCATATAAGC
		And
		5’GAGCCCTCAGATCCTGCATATAAGCAGCTGCTTTTTGCCTGTACTGGGTCTCTCTGGTTAGACCAGATCCGAGC
2	Del 1	5’TCGGAGGACAGTACTCCGACCCGGTCGAAGGGAGGCGTGGCCTGGGCGGGACTGAGCCCTCAGATCCTGCATATAAGC
		And
		5’GAGCCCTCAGATCCTGCATATAAGCAGCTGCTTTTTGCCTGTACTGGGTCTCTCTGGTTAGACCAGATCCGAGC
3	Del 2	5’TCGGAGGACAGTACTCCGACCCGGTCGAAGGGAGGCGTGGCCTTGGGGAGTGGCGAGCCCTCAGATCCTGCATATAA
		And
		5’GAGCCCTCAGATCCTGCATATAAGCAGCTGCTTTTTGCCTGTACTGGGTCTCTCTGGTTAGACCAGATCCGAGC

PCR primers used for amplifying all the constructs: Fwd TGTGAAGCTTTCGGAGGACAGTACTC, Rev: TGTGGAGCTCGGATCTGGTCTAAC.