Figure 1.

Polymerization cycles used

Code	Polymerization cycle
H1	at 74ºC, for 9 h			(Conventional)
H2	at 74ºC, for 9 h	+	at 100ºC, for 30 min	(Short-term terminal boil)
H3	at 74ºC, for 9 h	+	at 100ºC, for 3 h	(Long-term terminal boil)
H4a	at 74ºC, for 30 min	+	at 100ºC, for 30 min	(Short-term polymerization)

^a recommended polymerization cycle by the manufacturer