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. Author manuscript; available in PMC: 2015 Oct 23.
Published in final edited form as: Cell Rep. 2014 Oct 16;9(2):591–604. doi: 10.1016/j.celrep.2014.09.032

Figure 5. Recruitment of monocytes and differentiation into macrophages are required for development of joint pathology during STIA.

Figure 5

A Systemic treatment with DT eliminated blood monocytes while sparing neutrophils. Representative contour plots are shown. Numbers represent percentages of CD45+ cells. B. Systemic treatment with DT eliminates DTR-expressing tissue macrophages (CD45.2) in the synovium of CD11b-DTR(CD45.2)→CD45.1 bone marrow chimeras. Representative contour plots are shown and numbers indicate percentages of CD45+ cells. C. Repeated administration of DT decreases severity of arthritis (n = 10). D. Continuous treatment with DT decreased number of cells recruited to the joint (n=4 per time point). E. Dynamic of myeloid cell subsets in the joints during the course of STIA in PBS and DT-treated mice. F. Depletion of tissue macrophages during effector phase of STIA decreases joint damage. Top panel: hematoxylin and eosin staining (tibiotalar joint is shown), scale bar represents 100 μm. BM – bone marrow, C – cartilage, JC – joint cavity, P – pannus, SL – synovial lining. Bottom panel: immunohistochemical staining for macrophage marker F4/80. Arrows indicate F4/80-positive cells. Scale bar 20 μm. G. Depletion of tissue macrophages decreases histopathological scores. Data are represented as mean ± SEM. Differences between groups were compared using two-way ANOVA for repeated measurements, with Bonferroni post-test, * p<0.05, ** p <0.01, *** p<0.001.