Figure 7.

Lymphocyte activation depends on partial gravity exposure. Cells were treated after an adaptation period of 60 to 90 min with an activator solution and then cultured for 20 to 22 h on the RPI. Three different algorithms for partial gravity simulation (see Materials and Methods) were compared in the following order: Two Velocities, Flexible Static Intervals, and Fixed Static Intervals. The 1 g samples of each experiment were considered as 100% activated, and were used to standardize the other samples among the different experiments. A non-activated sample was used as a negative control. Means ± standard deviations were obtained from three independent series of experiments.