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. 2014 Oct 24;8:24. doi: 10.1186/1754-1611-8-24

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© Moustafa et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Effect of substrate stiffness. (A) A bright field optical image of human dermal fibroblasts after 6 days on 10:1 PDMS forming an interconnected network around 150 μm squares of PEG-DA. Phalloidin and DAPI stained cells on (B) 10:1 PDMS (C) 20:1 PDMS and (D) 5:1 PDMS shown with the nuclei and cytoskeleton overlaid. (Scale bar on all images = 200 μm).