Table 2.
Effect of CTNav1.5 mutations on steady state activation and inactivation.
| Activation | Inactivation | Recovery from inactivation |
|||
|---|---|---|---|---|---|
| V0.5 (mV) | K | V0.5 (mV) | K | tfast (sec) | |
| WTa | −42.8±1.2 (12) |
5.49±0.4 | −83.0±1 (19) | 3.35±0.1 | 5.5±0.5 (10) |
| Q1909Ra | −34.3±1.4 (9)* |
3.81±0.2* | −80.6±2.7 (8) | 2.96±0.1* | 5.1±0.7 (7) |
| R1910Aa | −43.2±2.2 (7) |
5.63±0.6 | −86.0±2.1 (6) | 3.60±0.1 | 6.0±0.5 (6) |
| R1910Ea | −41.4±4.5(3) | 5.53±1.5 | −79.7±2 (3) | 3.22±0.3 | 4.80±0.8(3) |
| K1922Aa | −36.2±1.3(4) * |
4.09±0.3 | −80.9±3.1 (4) | 3.42±0.3 | 5.20±0.8(4) |
| K1922Ea | −44.9±2.0 (6) |
5.46±0.3 | −78.4±1.7(3) | 3.55±0.3 | 6.21±1.7(4) |
| R1914Aa | −39.8±1.9(8) | 4.33±0.3* | −82.2±3(6) | 3.14±0.2 | 5.8±0.5(5) |
| R1914Ea | −39.0±2.5 (5) |
4.50±0.6 | −79.4±3.7(4) | 2.97±0.1* | 5.38±1.5(4) |
| WTa | −36 ± 2 (7) | −82.6±0.1 (5) | 3.8±0.4 (5) | ||
| WTb | −38 ± 2 (10) | −87.5±0.2 (14) | 5.5±0.1 (9) | ||
| D1790Ga,c | −38.9±0.5 (6) |
−96.5±0.1 (6) | 3.5±0.3 (5) | ||
| 4XD1790Ga,c | −37.4±1.7 (6) |
−99.9±0.1 (6) | 14.9±0.2 (7) | ||
| 4X- IQ/AAa,c |
−32.3 ± 2 (18) |
−100.8 ± 0.3 (9) |
18.2±0.5 (6) | ||
| D1885b | −34.1 ± 0.8 (18) |
−93.3 ± 0.1 (18) |
5.3±0.1 (9) | ||
| IQ/AAb | −32.7±2.6 (8) |
−82.5±0.4 (8) | 7.8±1.3 (7) | ||
Values are means SE (n).
a
in the presence of 0.5 mM/L Ca2+;
b
are with nominally Ca2+-free.
c
4X represents the mutations E1788A/D1790A/D1792A/E1799A published in Biswas et al.
*
denotes p<0.05 compared to WT.