. 2013 Oct 7;74(6):848–861. doi: 10.1002/ana.23974

Table 3.

Quantitative Data on Iron, Iron-Related Proteins, Cellular Markers, and Oxidative Damage

Staining	Quantification Method	NWM	NAWM	PPWM	Early Active	Late Active	Inactive	Remyelinated
Total nonheme iron	Ds	0.13 (0.62)	0.03 (0.7)^a	0.22 (2.37)^b	0.09 (3.45)	0.61 (3.04)	0.07 (0.53)	0.03 (1.09)
Ferritin	Ds	0.89 (2.23)	1.47 (3.89)	3.41 (13.54)^a^,^b	11.26 (9.11)^a^,^b	4.69 (13.07)^a^,^b	1.06 (4.7)	2.32 (3.07)^a^,^b
FTL	Ds	0.2 (1.13)	0.25 (1.95)	1.5 (14.53)^a^,^b	1.78 (13.62)^a^,^b	1.6 (15.23)	0.22 (1.5)	0.23 (2.54)
Oligodendrocytes
TPPP	Ct of TPPP⁺ cells	283.5 (399)	163 (452)	140 (407)	66 (215)^a^,^b^,^c		0 (0)^a^,^b	ND
Ferritin⁺ OG	Ct^a^,^b	128.5 (188)	98.83 (180)	24.5 (207)^a^,^b	1.67 (48)^a^,^b	1 (6)^a^,^b	0 (53)^a^,^b	41 (166)
FTL⁺ OG	Ct^a^,^b	58 (225)	50.5 (157)	9.5 (217)^a^,^b	2 (18)^a^,^b	1.17 (9)^a^,^b	0 (56)^a^,^b	18 (188)
Heph⁺ OG	Ct of TPPP^a^,^cHeph⁺ cells	27.5 (25)	14.5 (22)^a	24.5 (55)^b	5 (23)^a^,^c		0 (0)^a^,^b	ND
% Heph⁺ OG	TPPP⁺Heph⁺ cells / TPPP⁺ cells × 100	11 (16)	7 (12)	18 (26)^b	10 (10)^c		NA	ND
Microglia/macrophages
Iba-1	Ds	1.69 (2.28)	1.54 (4.13)	3.72 (13.42)^a^,^b	8.5 (7.81)^a^,^b	4.32 (7.77)^a^,^b	0.83 (3.1)	1.3 (4.16)
CD68	Ds	1.39 (1.68)	1.89 (5.08)	3.74 (19.32)^a^,^b	9.7 (12.55)^a^,^b	5 (7.84)^a^,^b	1.14 (6.4)	1.56 (3.17)
Iba-1	Ct	95.5 (155)	88 (285)	217 (824)^a^,^b	573 (620.33)^a^,^b	175 (502)^a^,^b	38 (283)^a^,^b	94 (187)
CD68	Ct	88.5 (45)	113 (153)	235.33 (602)^a^,^b	424.5 (522)^a^,^b	306 (369)^a^,^b	61 (259)^a^,^b	108 (120)
Ferritin⁺ MG	Ct	26 (34)	30 (97.5)	128.89 (568)^a^,^b	425 (477)^a^,^b	147 (504)^a^,^b	17.5 (108)	36 (76)
FTL⁺ MG	Ct	18 (35)	18 (78)	87.5 (359)^a^,^b	334 (321.5)^a^,^b	148.42 (436)^a^,^b	6 (69)	40 (89)
Ferritin⁺ Dys MG	Ct	1.5 (14)	4 (36)^a	19 (117)^a^,^b	14 (58)^a^,^b	10 (27)^a	9.5 (52)^a	3 (8)
FTL⁺ Dys MG	Ct	0 (9)	1.69 (19.5)	12.94 (65)^a^,^b	7.25 (10)^a^,^b	9 (38.33)^a^,^b	3.5 (45.5)^a	1 (7)
Astrocytes
Ferritin⁺ AG	Ct	0 (0)	0 (6)	2.88 (40)^a^,^b	2 (17)^a^,^b	17.5 (44)^a^,^b	15.5 (81)^a^,^b	4 (12)^a^,^b
FTL⁺ AG	Ct	0 (0)	0 (4)^a	2 (20.33)^a^,^b	0.83 (38)^a^,^b	4.5 (57)^a^,^b	4 (64)^a^,^b	1 (14)^a^,^b
Heph⁺ AG	Ct of GFAP⁺Heph⁺ cells	18 (30)	2 (15)^a	7.50 (40)	12 (67)^b^,^c		4 (9)	ND
Oxidative damage
E06⁺ axonal spheroids	Ct	0 (0)	0 (2)^a	2 (15)^a^,^b	5.25 (10)^a^,^b	1 (6)^a	0 (5)^a	0 (1)

Quantitative data are given as median (range in parentheses).

Significant versus NWM.

Significant versus NAWM.

Indicates no discrimination between early and late active regions of interest in the quantification.

AG = astroglia; Ct = manual counting; Ds = densitometry; Dys MG = dystrophic microglia; FTL = ferritin light polypeptide; GFAP = glial fibrillary acidic protein; Heph = hephaestin; MG = microglia/macrophages; NA = not applicable; NAWM = normal-appearing WM; ND = not determined; NWM = normal WM; OG = oligodendroglia; PPWM = periplaque WM; TPPP = tubulin-polymerization promoting protein; WM = white matter.