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. 2013 Sep 21;68(10):1269–1277. doi: 10.1111/all.12229

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© 2013 The Authors. Allergy published by John Wiley & Sons Ltd

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Concentration and time dependency of the inhibitory effect of the Cross-reactive carbohydrate determinants (CCD) blocker. ELISA experiments were conducted with an undiluted pool of 5 human CCD-positive sera. The coat antigen was ascorbate oxidase. The arrow indicates the concentration recommended for routine applications. The left panel shows the inhibition percentage in relation to inhibitor concentration (with 15-min pre-incubation). The right panel shows the effect of pre-incubation of serum with inhibitor (at room temperature with 20 μg/ml CCD blocker).