Table 2.
Cross-reactive carbohydrate determinants (CCD) inhibition in different test formats. The Mediwiss strip test (MW), the classical ImmunoCAP system and the ImmunoCAP ISAC allergen array were used to measure specific IgE against selected allergens without (n) and with (i) CCD blocker. The evaluation row ‘Eval’ shows ‘o.k.’ for supposedly correct inhibition below the 0.35 U/ml threshold. ‘+o.k.’ denotes values that had remained correctly positive despite CCD inhibition. ‘ins’ denotes insufficient inhibition, and ‘unex’ marks unexpected reduction in readings in case of supposedly CCD-free allergen. More examples are shown in Table S1
| m46 Allergen source | MW n U/ml | MW i U/ml | Eval | CAP n U/ml | CAP i U/ml | Eval | Component | ISAC n ISU-E | ISAC i ISU-E | Eval |
|---|---|---|---|---|---|---|---|---|---|---|
| Alder pollen | 44.1 | 0 | o.k. | |||||||
| Birch pollen | >100 | 0 | o.k. | 19.6 | 0.11 | o.k. | rBet v 1/2/4 | 0/0/0 | 0/0/0 | |
| Hazel pollen | >100 | 0.23 | o.k. | |||||||
| Grass pollen mix | 99.7 | 0 | o.k. | 25.0 | 1.11 | ins | rPhl p1/2/5/6/7/11/12 | 0/0/0/0/0/0/0 | 0/0/0/0/0/0/0 | |
| Rye pollen | >100 | 0 | o.k. | nPhl p4*/nCyn d 1* | 10/26 | 0/0 | o.k | |||
| Mugwort pollen | 14.9 | 0 | o.k. | 21.6 | 0.21 | o.k | nArt v1 | 0 | 0 | |
| Ragweed pollen | >100 | 0 | o.k. | 25.7 | 0.49 | ins | nAmb a1 | 0 | 0 | |
| Plantain pollen | 32.9 | 0 | o.k. | rPla l 1 | 0 | 0 | ||||
| Mite (D. pteronyssinus) | 0.59 | 0 | rDer p 1/nDer p2 | 0/0 | 0/0 | |||||
| Mite (D. farinae) | 0.48 | 0 | ||||||||
| Cockroach | 13.6 | 0 | o.k. | rBla g 1/2/5/7 | 0/0/0/0 | 0/0/0/0 | ||||
| Cat | 0 | 0 | rFel d 1 | 0 | 0 | |||||
| Dog | 0.37 | 0 | unex | rCan f 1 | 0 | 0 | ||||
| Hazelnut | 3.4 | 0 | o.k. | 17.4 | 0.08 | o.k | rCor a 1/8 | 0 | 0 | |
| Peanut | 1.7 | 0 | o.k. | rAra h 1/2/3/8/9 | 0 | 0 | ||||
| Walnut | 6.4 | 0 | o.k. | nJug r 1/2*/3 | 0/10/0 | 0/0/0 | o.k. | |||
| Wheat flour | 42.3 | 0 | o.k. | rTri a 14/19 | 0/0 | 0/0 | ||||
| Rye flour | 73.1 | 0 | o.k. | |||||||
| Soy | 1.4 | 0 | o.k. | rGly m 4/nGly m 5/6 | 0/0/0 | 0/0/0 | ||||
| Apple | 1.8 | 0 | o.k. | rPru p 1/3 | 0/0 | 0/0 | ||||
| Celery | 27.1 | 0 | o.k. | rApi g 1 | 0 | 0 | ||||
| Bee venom | 28.5 | 1.1 | (o.k.) | |||||||
| Component: rApi m1 | 1.63 | 0.31 | ?a | rApi m1 | 0 | 0 | ||||
| Yellow jacket venom | 28.5 | 18.3 | +o.k. | rPol d5 | 1.0 | 1.5 | +o.k | |||
| Component: rVes v 1/5 | 11.8/48.7 | 9.7/44.6 | +o.k. | rVesv5 | 6.2 | 8.9 | +o.k | |||
| CCD mix | >100 | 0 | o.k. | CCD MUXF3 | 20 | 0 | o.k. | |||
| CCD bromelain | 38.4 | 0 | o.k. | nCry j 1* | 9.3 | 0 | o.k. | |||
| CCD HR-peroxidase | 9.3 | 0 | o.k. | nCup a 1* | 11 | 0 | o.k. | |||
| CCD Asc-oxidase | 30.4 | 0 | o.k. | nOle e 1* | 4.5 | 0 | o.k. | |||
| nPla a 1* | 12 | 0 | o.k. |
Conflict between positive CAP and negative ISAC of Api m1.
Natural components, most probably with CCD-type glycosylation.