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Antibodies and conjugates used for double labeling
| Antigen | Species | Final dilution | Source | Secondary ab’s and conjugates |
|---|---|---|---|---|
| Human Iba1 | Rabbit | 1:2000 | WAKO chemicals | DoaR-AF488 1:400 |
| Cow GFAP | Rabbit | 1:2000 | DAKO | DoaR-AF488 1:400 |
| Human CD68 | Mouse | 1:300 | DAKO, clone KP1 | DoaM-AF488 1:400 |
| Human TLR2 | Goat | 1:500 | R&D systems | DoaG-AF594 1:400 |
