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. 2014 Nov 1;17(11):1197–1203. doi: 10.1089/jmf.2013.3091

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Copyright 2014, Mary Ann Liebert, Inc. and Korean Society of Food Science and Nutrition

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FIG. 3. — Inhibition of Takeda G-protein-coupled receptor 5 (TGR5) mRNA expression by small interference RNA (siRNA), and effect on AEE-SM-induced GLP-1 release and intracellular Ca²⁺ and cAMP increases from NCI-H716 cells. Transfections using nontargeting siRNA were used as controls. (A) TGR5 mRNA assessed by real-time quantitative PCR 48 h posttransfection. (B) Effect of TGR5 knockdown on AEE-SM-induced GLP-1 release. (C) Effect of TGR5 knockdown on AEE-SM-induced increases in intracellular Ca²⁺ levels. (D) Effect of TGR5 knockdown on AEE-SM-induced increases in intracellular cAMP levels. The data are mean±SEM (n=12); values not sharing a common letter are significantly different (P<.05 by ANOVA and Tukey's test). *P<.05, **P<.01, ***P<.001 versus control siRNA.