FIG. 4.

ROS deficient Ncf1^m1J mutated mice have a reduced total memory B-cell compartment. Flow cytometry analysis of heparinized peripheral blood from the Ncf1^m1J mutated and BQ wild-type mice was performed to determine B-cell subset frequencies. The frequency of total B cells (CD19⁺) was calculated within the total lymphocyte gate based on FSCxSSC. Total memory B cells (CD19⁺CD23⁺CD21⁺IgM^+/−IgD⁻) and naïve B cells (CD19⁺IgM⁺IgD⁺⁺) were calculated within total B cells. B cells expressing activation marker CD69 (CD19⁺CD69⁺), homing receptor (CD19⁺CXCR4⁺), or the apoptosis-associated Fas receptor (CD19CD95⁺) were calculated within total B cells. Mean±SEM, n=6/group, two-way Mann–Whitney test.