Skip to main content
. 2014 Nov 7;5:628. doi: 10.3389/fpls.2014.00628

Table 2.

Frequency of transformation in cereals and grasses mediated by Agrobacterium tumefaciens.

Species, Genotype Target explant Independent events explant Reference
Rice (Oryza sativa L.), japonica (eg. Nipponbare, Koshihikari) Immature embryo 18.0* Hiei and Komari (2008)
Rice (Oryza sativa L.), indica (eg. IR64, IR72) Immature embryo 15.0* Hiei and Komari (2008)
Rice (Oryza sativa L.), japonica (eg. Nipponbare, Koshihikari) Callus 0.9 Hiei and Komari (2008)
Maize (Zea mays L.), A188 Immature embryo 0.5 Ishida et al. (2007)
Wheat (Triticum aestivum L.), Fielder Immature embryo 0.9 Ishida et al. (2014)
Barley (Hordeum vulgare L.), Golden Promise Immature embryo 0.87 Hensel et al. (2008)
Sorghum (Sorghum bicolor L.), Tx430 Immature embryo 0.33 Wu et al. (2014)
Rye (Secale cereale L.) Immature embryo 0.03 Popelka and Altpeter (2003)
Oats (Avena sativa L.) Immature embryo 0.12 Gasparis et al. (2008)
Fox tail millet (Setaria italica L.) Callus 0.06 Wang et al. (2011)
Finger millet (Eleusine coracana (L.) Gaertn.) Callus 0.04 Antony Ceasar and Ignacimuthu (2011)
Pearl millet [Pennisetum glaucum (L.) R. Br.] Callus 0.03 Ramadevi et al. (2014)
Sugar cane (Saccharum officinarum L.) Pre-cultured seed 0.45 Mayavan et al. (2013)
Bermuda grass (Cynodon dactylon L.) Callus 0.05 Salehi et al. (2005)
Bentgrass (Agrostis stolonifera L.) Callus 0.03 Yu et al. (2000)
Italian Ryegrass (Lolium multiflorum Lam) Callus 0.07 Lee et al. (2010)
Perennial Ryegrass (Lolium perenne L.) Callus 0.2 Patel et al. (2013)
Tall fescue (Festuca arundinacea Schreb.) Callus 0.08 Dong and Qu (2005)
Zoysia japonica Steud. Stolon node 0.07 Ge et al. (2006)
Switch grass (Panicum virgatum L.) Callus 1.0 Li and Qu (2011)
Chinese silvergrass (Miscanthus sinensis Andersson) Callus 0.01 Hwang et al. (2014)
Brachypodium distachyon (L.) Callus 0.67 Steinwand et al. (2013)

High end values found in the literature are displayed. *The explants were sectioned into pieces after the co-cultivation so that many transgenic events were recovered from a single original explant.