Table 1.

Significant Effects of Vandetanib and Doxorubicin Alone and in Combination on the Phosphorylation of Different Cell Signaling Pathways as Observed by Western Blot Analyses (24 h)

Cells	Treatment	pERK1	pERK2	pERK5*	pCREB*	pAKT	pEGFR
HMESO	Van 5 μM				↓†
	Dox 25 μM	↑	↑	↑	↑	↑	↓
	Van 5 μM + Dox 25 μM‡	↓	↓	↓	↓	↓
H2373	Van 5 μM
	Dox 100 μM	↑	↑	↑	↑	↑	↓
	Van 5 μM + Dox 100 μM	↓	↓	↓	↓	↑	↓

Definition of abbreviations: Dox, doxorubicin; pAKT, phosphorylated AKT; pCREB, phosphorylated cyclic AMP response element binding protein; pEGFR, phosphorylated epidermal growth factor receptor; pERK, phosphorylated extracellular signal-regulated kinase; Van, andetanib.

^*pERK5 and pCREB present novel findings for this study.

^†Arrows represent P < 0.05 increases (up arrows) or decreases (down arrows).

^‡All comparisons with Van + Dox are with the Dox alone group. Comparisons involving individual agents are with the control group.