Figure 7.

Glucose deprivation alters the levels of sense and antisense hexose transporter transcripts in wild-type cells. HXT sense and antisense transcript abundance using strand-specific RT–qPCR in wild-type cells grown in glucose or after glucose depletion. Wild-type cells were isolated following growth in glucose (2%) or after a 10-min shift to glucose-depleted media. The levels of (A) sense and (B) antisense hexose transporter transcripts were determined by strand-specific RT–qPCR as in Figure 4 and are reported as the average of three biological replicates with the SEM.