Table 2. Oligonucleotides for Chromatin Immunoprecipitation (ChIP).
| Name | Forward primer | Reverse primer | Probe |
|---|---|---|---|
| HXT1 antisense | TTCCAGGCTGTCGGTTTAAG | AGCACCCCACATCAAACAG | CCAAAACGGTCAACGGTGTAC |
| HXT1 sense | GGCCATGAATACTCCAGAAGG | CACCGAAAGCAACCATAACAC | AGTGAAAGTCAAGTGCAACCCGC |
| HXT4 sense | GTTGGTGTTACAAGATTGTGGC | CAGGTAGTGGCAAAACAGAATAAG | AACGGGTCTTCTAAGGGTGCTGG |
| HXT5 antisense | TTACTCGAGGTTTCAACAGGG | AGGTAGCGGAGTTTTCAGTTC | AATCAAGAGCCCCGTTCTTTTACCGT |
| HXT5 sense | CGGAACTTGAAAACGCTCATC | TGAGACGGGTTTAGCTTGTG | CCTTGGAAGGGTCTGCTACTGTGA |
| HXT8 antisense | TCTGTTGATAAGTTGGGCCG | GTAAATAACCATGCACGCCG | TCTTTTACTTGGAGCAGCCACCATGA |
| HXT8 sense | TTAGTGTTCTTGCCCCGATG | CGAAAGTCACCATCAATTGCC | ACTGCGCCAAAGCATATCAGAGGT |
Oligonucleotides for ChIP are Primetime qPCR assays (IDT) and are listed as forward and reverse primers and probe for each gene tested.