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A monomer of clone-601 DNA (207 bp NRL) placed between the silencer and the reporter gene does not block silencing. Arrows indicate 207 bp clone-601 monomers placed in different orientations between the HML–E silencer (A and B) or the HML–I silencer (C and D) elements and the URA3 reporter gene. The URA3 reporter status was tested by spotting assay.
