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An array of 12 regular repeats of clone-601 nucleosomes (207 bp NRL) blocks silencing of the URA3 reporter. Arrows indicate 207 bp clone-601 oligomers (regular 601-207×12 repeats) placed in different orientations between the HML–E silencer (A and B) or the HML–I silencer (C and D) elements and the URA3 reporter gene. The URA3 reporter status was tested by spotting assay.
