Figure 6.
The 207 bp NRL arrays of up to six nucleosomes conduct silencing of the reporter gene by the E- or I-silencer elements. (A–E) The repeats of clone-601 DNA with 207 bp-long NRL (shown by open arrows) were positioned between the HML–E silencer and the URA3 reporter. The URA3 reporter status was tested by spotting assay. (F–J) The repeats of clone-601 DNA with 207 bp-long NRL (shown by open arrows) were positioned between the HML–I silencer and the URA3 reporter. The URA3 reporter status was tested by spotting assay.