Figure 6.

The Hippo pathway controls the expression of upd in polar cells. GFP-negative mitotic clones were generated in FRT42D (A), FRT42D hpo^42-47 (B), and FRT42D yki^B5 (C) in an upd-lacZ background. The ovaries were immunostained with anti-Fas3 and anti-β-galactosidase (β-Gal). Stage-9 and stage-10 egg chambers were selected and oriented as anterior toward the left. High magnification views of border cell clusters are shown on the right. (A) An FRT42D control polar cell (yellow arrowheads) was positive for β-Gal as the neighboring GFP-positive polar cell (white arrowheads). (B) A yki mutant polar cell (yellow arrowheads) showed a higher β-Gal level than that of the neighboring GFP-positive polar cell (white arrowheads). (C) A hpo mutant polar cell (yellow arrowheads) showed a lower β-Gal level than that of the neighboring GFP-positive polar cell (white arrowheads). (D) Quantification of β-Gal levels in FRT42D, yki, and hpo mutant polar cells in an upd-lacZ background. The bar graph is shown as mean ± SEM. Paired Student’s t-test, **P < 0.01. Bar, 20 μm in A–C.