Figure 4.
Overexpression/hyperactivation of pathway components does not enhance filamentous growth. (A) Plate-washing assay was performed for the indicated strains on YEP–GAL. GAL–MSB2 and GAL–SHO1 strains where the GAL1 promoter has replaced the native promoter. MSB2Δ100–818 is a hyperactive allele of MSB2. (B) Septin staining. Strains containing the pCdc12p–GFP plasmid were grown on SCD or S medium lacking glucose. Bar, 5 μm. (C) Indicated strains were stained with rhodamine phalloidin. Bar, 5 μm. (D) Colocalization of Sho1p and Cdc24p in cells carrying a hyperactive allele of MSB2. Bar, 5 μm.