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Effect of AnTc on revertant yield requires Tn10dTc and exposure during pregrowth. The strain without Tn10dTc (TR7178) is the original tester strain of Cairns and Foster (1991) and the strain with Tn10dTc in lac (TT26327) carried an insertion at the distal end of the lac operon of the F′128 plasmid. Cells were pregrown for 20 generations in minimal glycerol with and without AnTc (216 nM) and plated on lactose medium with or without AnTc.
