Figure 7.

Effect of Tn10dTc position on sensitivity of reversion to AnTc. Cells were pregrown to stationary phase in glycerol with or without AnTc (216 nM) and then washed and plated on selective lactose plates with or without AnTc (216 nM). The strain at the top carries Tn10dTc in the chromosomal btuR gene (TT26328). The strain at the bottom carries Tn10dTc in the yebB gene (TT26323). Revertant number is expressed relative to viable cells plated. This normalization understates the effect of pregrowth with AnTc, by including only cells that have survived the effects of AnTc and eliminating those multicopy cells lost during pregrowth.