Figure 5.

ChR2-EYFP-positive large cortical terminals from barrel cortex innervate POm. (A) Injection site of the anterograde tracer PHAL into the mouse somatosensory cortex. (B) Low-power image of the anterogradely labeled PHAL-containing cortical fibers in the thalamus. Asterisk denotes the location of high-power images (C). (C) Large ChR2-EYFP-positive terminals (arrows and open arrowheads, top) co-localize with PHAL-labeled terminals (orange arrows, middle and bottom). As EYFP is expressed only in layer 5 cells, the co-localization demonstrates that ChR2-positive cortical L5 neurons project to POm with large driver terminals. Open arrowheads, EYFP-positive, PHAL-negative terminals; filled arrowheads, EYFP-negative, PHAL-positive terminals. Scale bar, A: 1 mm; B: 500 μm; C: 5 μm.