Fig. 2.—

Identification of monotreme MLP by SDS-PAGE and mass spectrometry. (A) Coomassie stained SDS-PAGE gel and ESI-TOF mass spectrometry identifying MLP of platypus (asterisk) and short-beaked echidna (arrow) in milk. Lane 1: molecular weight marker, lane 2: platypus skim fraction, and lane 3: echidna skim fraction. (B) The complete amino acid sequence of platypus MLP and (C) short-beaked echidna MLP are shown overlaid with identified peptides (bold and underlined) showing sequence coverage of 29% and 27%, respectively.