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. 2014 Nov 7;9(11):e110844. doi: 10.1371/journal.pone.0110844

Figure 1. Nutlin-3 causes a p53-dependent tetraploid G1-arrest in diploid HCT116 clones.

Figure 1

A) HCT116 diploid clones D3 and D8, and HCT116 that express wild-type p53 (p53+/+) or are p53-null (p53-/-) were untreated or treated with Nutlin (NUT 10 µM) for 24 hrs, and cell cycle profile determined by flow cytometry. B) Expression of the indicated proteins was monitored by immunoblot in cells untreated or treated with Nutlin (NUT 10 µM) for 24 hrs. C) mRNA levels for the indicated factors in untreated cells or cells treated with Nutlin (NUT 10 µM) for 24 hrs was determined by qRT-PCR.