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. 2014 Nov 7;9(11):e112138. doi: 10.1371/journal.pone.0112138

Figure 2. PVN monosynaptic glutamatergic projections to CVNs.

Figure 2

A, Schematic of approach, left. Right, postsynaptic responses were completely blocked by antagonists for NMDA (AP-5; 50 µM) and AMPA/kainate receptors (CNQX; 50 µM). B, Paired-pulse facilitation (10 Hz) of glutamatergic neurotransmission from PVN to CVNs (first trace) abolished by oxytocin antagonist OTA (10 µM; middle trace) and partly restored upon wash out (right trace) C, Average data of OTA abolished paired-pulse facilitation (n = 7). * p = 0.021. D, Five consecutive stimulations (10 Hz;) with increasing amplitudes (black trace). OTA reduced the amplitude (red trace). Traces are normalized to amplitude of first response. E, Average data of the five stimulation paradigm (* p = 0.014)).