Table 1. Treatments with microconidia concentrations and temperatures.
iTRAQ 8-plex tags | Microconidia concentration and temperatures | Volume recovered |
113 | Hemolymph challenge with F.oxysporum 104 microconidia/mL at 25°C | 6 mL |
114 | Hemolymph injected only with Tween-80 (0.1% v/v) at 25°C | 6 mL |
115 | Hemolymph challenge with F.oxysporum 104 microconidia/mL at 37°C | 6 mL |
116 | Hemolymph untreated at 25°C | 6 mL |
117 | Hemolymph challenge with F.oxysporum 106 microconidia/mL at 37°C | 6 mL |
118 | Hemolymph injected only with Tween-80 (0.1% v/v) at 37°C | 6 mL |
Sample tags for iTRAQ analysis. For each treatment or pool around 6 mL were recovered. The protein concentration was calculated by Bradford assay.