Figure 2. The pathogenic rtPCR does not amplify DNA from cultured L. biflexa isolates.

Amplification curves for cultured isolates of L. interrogans (solid diamonds) and L. biflexa (open circles) in the (A) pathogenic rtPCR and (B) UFI assay. Results are displayed for two isolates of each species. The threshold for positivity is set at 0.05 normalized fluorescence units for both assays (dotted red line).