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. 2014 Nov 7;9(11):e112356. doi: 10.1371/journal.pone.0112356

Figure 4. The pathogenic rtPCR shows blunted amplification of a clinical sample with 16S sequence consistent with a non-pathogenic Leptospira strain.

Figure 4

Amplification curves for a clinical sample with sequence matching the non-pathogenic Leptospira, L. meyeri, L. biflexa, and L. wolbachii (open circles) in the (A) pathogenic rtPCR and (B) UFI assay. Two clinical samples with 16S sequence matching pathogenic strains (solid diamonds) are shown for comparison. The threshold for positivity is set at 0.05 normalized fluorescence units for both assays (dotted red line).