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. 2014 Nov 7;9(11):e112356. doi: 10.1371/journal.pone.0112356

Table 3. Pairwise comparisons of Leptospira PCR diagnostics.

Pathogenic rtPCR
Positive Negative Total
UFI Assay Positive 62 3 65
Negative 0 0 0
Reference 16S rtPCR Positive 24 0 24
Negative 39 2 41
flaB/lipL41 Positive 15 1 16
Negative 47 2 49
Composite Reference Positive 28 1 29
Negative 34 2 36

Comparison of the pathogenic rtPCR with the UFI assay, reference 16S rtPCR, conventional PCRs for flaB/lipL41, and a composite reference that takes into account flaB/lipL41 and the reference 16S rtPCR. Samples that tested positive by at least one of these assays were considered positive, while those that tested negative by both assays were considered negative for this composite reference. Samples that tested negative in the pathogenic rtPCR had CT values of 34.77, 35.26, and 36.11 in the UFI assay.