Table 3. Pairwise comparisons of Leptospira PCR diagnostics.

		Pathogenic rtPCR
		Positive	Negative	Total
UFI Assay	Positive	62	3	65
	Negative	0	0	0
Reference 16S rtPCR	Positive	24	0	24
	Negative	39	2	41
flaB/lipL41	Positive	15	1	16
	Negative	47	2	49
Composite Reference	Positive	28	1	29
	Negative	34	2	36

Comparison of the pathogenic rtPCR with the UFI assay, reference 16S rtPCR, conventional PCRs for flaB/lipL41, and a composite reference that takes into account flaB/lipL41 and the reference 16S rtPCR. Samples that tested positive by at least one of these assays were considered positive, while those that tested negative by both assays were considered negative for this composite reference. Samples that tested negative in the pathogenic rtPCR had C_T values of 34.77, 35.26, and 36.11 in the UFI assay.